America's Historical and Natural Legacy Study Act

America's Historical and Natural Legacy Study Act - (Sec. 3) Directs the Secretary of the Interior to conduct a special resource study of the Harry S Truman Birthplace State Historic Site in Lamar, Missouri, to determine: (1) the suitability and feasibility of adding the birthplace site to the Harry S Truman National Historic Site or designating the site as a separate unit of the National Park System; and (2) the methods and means for the protection and interpretation of the Harry S Truman Birthplace State Historic Site by the National Park Service (NPS), other federal, state, or local government entities, or private or nonprofit organizations. Requires submission of a report on the results of such study and any recommendations of the Secretary with respect to the birthplace site.

(Sec. 4) Directs the Secretary to conduct a special resource study of the sites and resources at Matewan, West Virginia, associated with the Battle of Matewan (the Matewan Massacre) of May 19, 1920, to determine: (1) the suitability and feasibility of designating certain historic areas of Matewan as a unit of the National Park System; and (2) the methods and means for the protection and interpretation of the historic areas by the NPS, other federal, state, or local government entities, or private or nonprofit organizations. Requires submission of a report on the results of such study and any recommendations of the Secretary with respect to the historic areas.

(Sec. 5) Directs the Secretary to conduct a special resource study along the Ox-Bow Route of the Butterfield Overland Trail in Missouri, Tennessee, Arkansas, Oklahoma, Texas, New Mexico, Arizona, and California to evaluate: (1) a range of alternatives for protecting and interpreting the resources of the route, including alternatives for potential addition of the Trail to the National Trails System; and (2) the methods and means for the protection and interpretation of the route by the NPS, other federal, state, or local government entities, or private or nonprofit organizations. Requires submission of a report on the results of such study and any recommendations of the Secretary with respect to the route.